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As an important substitute for agarwood, mountain-agarwood, belonging to the family Oleaceae, comes from the root, stem and thick branch of Syringa pinnatifolia, which has a wide range of application in Inner Mongolia, China. It has good clinical efficacy in the use of cardiovascular diseases. However, the formation speed of mountain-agarwood is extremely slow, and its cultivated seedlings have low resin content. Therefore, how to speed up the formation of mountain-agarwood and increase the resin content is a hot research topic in this field. In this work, 16 S rDNA amplicon sequencing method was used to systematically analyze the bacterial communities of different samples of mountain-agarwood. Our data revealed that the samples of mountain-agarwood had more obvious species diversity than the ones of non-mountain-agarwood, especially the wild mountain-agarwood samples. By analysis of bacterial community composition and species abundance, Sphingomonas, Modestobacter and unidentified Cyanobacteria genus were three dominant bacterial genera in all samples. In addition, there are two identified genera of dominant bacteria, namely Actinoplanes and Microbacterium in both wild and cultivated mountain-agarwood, by bacterial community composition and species richness analysis. Meanwhile, Roseomonas was the dominant bacterial genus in both wild and cultivated non-mountain-agarwood samples. Our work could provides basic data for exploring the mechanism of the mountain-agarwood formation, and help to exploit resource of endophytic bacteria reasonably.
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Bacteria , Genetics , China , DNA, Ribosomal , Resins, Plant , ThymelaeaceaeABSTRACT
To establish a robust and accuracy molecular method to identify Achyranthis Bidentatae Radix and Cyathulae Radix formula granules. ITS sequences of Achyranthes bidentata and Cyathula officinalis were aligned, specific SNPs (single nucleotide polymorphisms) were excavated, specific primers were designed and allele-specific PCR method was established. The genomic DNA was successfully extracted from the herbal medicine and its formula granules by using an improved CTAB (cetyltrimethyl ammonium bromide) method and then performed PCR with the designed primers. The 187 bp specific band could be amplified only in the presentation of C. officinalis and its granules when use of C. officinalis specific primers, whereas the 162 bp band could be amplified only in the presentation of A. bidentata and its granules when use of A. bidentata specific primers. This method was also successfully applied in the identification of commercial formula granules.
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BACKGROUND: Due to its strong catalytic activity and selective catalytic oxidation, gold-silver bimetallic nanoparticles have potential applications in biomedicine, drug delivery and electrochemical analysis.OBJECTIVE: To prepare the gold-silver alloy nanoparticles using mitochondria in animal cells as templates. METHODS: Separating mitochondria from the fish liver: 1 mL of mitochondrial mother liquor was added into 1 mL of alkaline HAuCl4solution (10 mmol/L) and shaken; then, 1 mL of AgNO3solution (10 mmol/L) was added, and the mixture was reacted in an electromagnetic stirrer for 20-30 hours until the color of the reaction solution was changed from colorless to purple, which indicated that gold and silver alloy nanoparticles were successfully obtained; finally, the characterization of the nanoparticles was analyzed. (1) Cytotoxicity test: gold-silver alloy nanoparticles atdifferent concentrations (0, 25, 50, 75, 100, 125, 150 mg/L) were added into gastric cancer cells. After 48 hours of culture, the proliferation of cells (absorbance value) was detected by MTT assay. (2) Stability evaluation: 1 mL of gold-silver alloy nanoparticles at different concentrations (0, 25, 50, 75, 100, 125, 150 mg/L) were placed into glass reaction flasks and then, 0.2 mL of ultrapure water was added in portions following by shaking. After addition of the ultra-pure water, ultraviolet-visible spectrum was used to analyze the characterization of the solution. RESULTS AND CONCLUSION: The gold-silver alloy nanoparticles were spherically distributed and confirmed to have an alloy structure with the average particle size of 20-30 nm, and presented to have the advantages of simple structure, good crystallinity, and active groups such as hydroxyl and carbonyl groups on the surface. MTT results showed that the gold-silver alloy nanoparticles at the mass concentration of < 150 mg/L had no obvious cytotoxicity. The characteristic absorption peak with the largest intensity was positively correlated with the concentration of gold-silver alloy nanoparticles.To conclude,the gold-silver alloy nanoparticles have good cytocompatibility and stability in vitro.
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AIM: To investigate the incidence of trachoma in children aged 1 to 9y in Hainan Province and determine high-risk trachoma endemic and non-endemic areas in Hainan, and thus provide evidence for developing trachoma control and prevention therapy.METHODS:The areas of investigation were chosen on the basis of past literatures, expert interviews and survey on the spot.In 2013, Hainan Provincial Office of Blindness Prevention carried out the survey in 7 counties including Dongfang City, Wuzhishan City, Ledong County, Baisha County, Baoting County, Lingao County and Changjiang County.In these districts, 356 pupils including 192 boys and 164 girls were examined, their age ranging from 1 to 9 and their average age being 7 years old.The targeted students received the trachoma rapid assessment by the adoption of simplified trachoma classification system which was recommended by the World Health Organization.RESULTS: No case of active trachoma was found among the 356 students.CONCLUSION:The prevalence rate of trachoma in children under 9 years is less than 5% in Hainan Province.Active trachoma is not a public health issue in Hainan Province.
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BACKGROUND: The study aimed to investigate the clinical characteristics of acute renal failure (ARF) caused by oral acyclovir. METHODS: A 45-year-old Chinese male patient with acyclovir-induced ARF suffered from abdominal pain for one day. The pain was extended to the epigastric area from the right lower quadrant. Transient oliguria was seen in addition to microscopic hematuria and proteinuria. The serum creatinine concentration was 304 μmol/L. Eight days before the occurrence of ARF, the patient took oral acyclovir for facial neuritis. RESULTS: His renal function was restored completely following the discontinuation of acyclovir, with continuous renal replacement therapy for 54 hours and some symptomatic treatment. CONCLUSION: The presentation of acute renal failure caused by acyclovir can be diverse, but the prognosis is good after active treatment.
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<p><b>OBJECTIVE</b>To analyze the change of the neuronal restricted silencing factor (NRSF) gene as well as the NRSF regulation genes in beta-mercaptoethanol induction of the marrow mesenchymal stem cells (MSCs) to neurons, and to discuss the function of NRSF in neural induction of the MSCs and the mechanism of the differentiation from MSCs to neurons.</p><p><b>METHOD</b>We used beta-mercaptoethanol, serum-free DMEM, and dimethyl sulfoxide to induce rat MSCs to differentiate to neurons, and then analyzed the changes of the expressions of NRSF gene and NRSF-regulated genes through real-time PCR.</p><p><b>RESULTS</b>The rat MSCs were successfully induced to differentiate into neuron-like cells. The induced neuron marker, neuron-specific enolase, was positive. Real-time PCR showed that the expression of NRSF gene remarkably declined. The expressions of neurotrophic tyrosine kinase receptor, type 3, synaptosomal-associated protein 25, L1 cell adhesion molecular,neuronal pentraxin receptor in the NRSF-regulated genes also increased at varied extents.</p><p><b>CONCLUSIONS</b>The differentiation from MSCs to neurons is relevant with the decline of NRSF expression and the increase of the expressions of NRSF-regulated genes. The NRSF may be the key gene during the differentiation from MSCs to neurons.</p>
Subject(s)
Animals , Rats , Bone Marrow Cells , Cell Biology , Metabolism , Cell Differentiation , Physiology , Cells, Cultured , Mesenchymal Stem Cells , Cell Biology , Metabolism , Neurons , Cell Biology , Metabolism , Phosphopyruvate Hydratase , Genetics , Metabolism , Rats, Wistar , Repressor Proteins , Metabolism , Physiology , Synaptosomal-Associated Protein 25 , Genetics , MetabolismABSTRACT
Objective To observe the therapeutic effect of Bushen Tongluo Pill (Pills for reinforcing kidney and removing obstruction of meridians) on rheumatoid arthritis (RA).Methods Totally 100 RA patients were randomized into treatment group and control group with 50 cases in each group.The treatment group (50 cases) was administered Bushen Tongluo Pill and the control group (50 cases) was prescribed Celecoxib capsules and Methotrexate tablets for 3 months.The changes after treatment in the clinical symptoms including joint pain,swelling,joint functions,hand grip strength,and in the laboratory indices including erythrocyte sedimentation rate (ESR),rheumatoid factors (RF),and C-reactive protein (CRP),and the untoward effect as well were observed.Results The total effective rate of the treatment group was higher than that of the control (P0.05).Conclusion Bushen Tongluo Pill is effective for RA,the effect was superior to that of Celecoxib capsules and Methotrexate tablets.